I actually did the opposite. I have it spread out with my intake to insure I do not have and dead spots, I have mine set up alternating. At the far left I have output, then intake, then output, then intake at the far right side.
It works good for me that way. an it seems like we all agree, coarse on bottom, bio in middle, fine sponge on top. But me being HUGE on bio media, I also have more bio in the top tray, you cant have too much! and then I topped that off with the fine sponge, that works perfectly fine. You dont have to put just one kind in a tray, adding bio with mechanical will be fine, just be sure on the top one that you put the fine sponge on TOP of the bio, if thats what you choose to do. I also kept nothigng but coarse sponge on the bottom, no bio there.