Welcome to the site
Is your tank cycled? I'm doubtful from what you've just said about just setting it up. Anyway, to answer your question, I assume we are talking about a water conditioner to remove chlorine? If you are using buckets to replace water then you can work out how much water you are taking out and then work out how much water conditioner you need. You can then add it to each bucket if you want. You don't need to test the water as most water conditioners remove chlorine instantly. Alternatively, put some water conditioner straight in your tank and then add your water.
The easiest way to refill your tank is using a hose pipe attached straight to your tap. Again, add the water conditioner into the tank as the water goes in, just before. I been doing it like this for years.